CoV-GLUEGenomeCountriesLineagesMutationsInsertionsDeletionsUpdated:

CoV-GLUE | Mutations | Mutation

CoV-GLUE Mutation: ORF = ORF1ab/nsp1 : CodonNum = 48

Details of the codons used for the mutation across all lineages (CodonProp is the proportion each codon is used for this AA mutation):

ORFCodonNumMutORF1abMutMutTypeRefCodonMutCodonAACountCodonCountCodonPropGenome Position
ORF1ab/nsp148D48GD48GnonsyngAtgGt159215921407-409
ORF1ab/nsp148D48ND48NnonsynGatAat102610261407-409
ORF1ab/nsp148D48DD48DsyngaTgaC4844841407-409
ORF1ab/nsp148D48VD48VnonsyngAtgTt3223221407-409
ORF1ab/nsp148D48YD48YnonsynGatTat2262261407-409
ORF1ab/nsp148D48ED48EnonsyngaTgaA116750.646552407-409
ORF1ab/nsp148D48ED48EnonsyngaTgaG116410.353448407-409
ORF1ab/nsp148D48delD48deldelGAT---98981407-409
ORF1ab/nsp148D48AD48AnonsyngAtgCt57571407-409
ORF1ab/nsp148D48HD48HnonsynGatCat441407-409
ORF1ab/nsp148D48TD48TnonsynGATACA331407-409
ORF1ab/nsp148D48del1D48del1delGat-at210.5407-409
ORF1ab/nsp148D48del1D48del1delgaTga-210.5407-409
ORF1ab/nsp148D48CD48CnonsynGATTGC111407-409
ORF1ab/nsp148D48del2D48del2delgATg--111407-409
ORF1ab/nsp148D48LD48LnonsynGATTTG111407-409

Details of the mutation no within lineages (LineageCodonProp is the proportion the codon is observed at, at this particular site, on each lineage):

ORFCodonNumMutRefCodonMutCodonCodonCountAACountLineageLineageCodonProp
ORF1ab/nsp148D48GgAtgGt15921592All0.000304
ORF1ab/nsp148D48GgAtgGt12101210B.1.177.170.993432
ORF1ab/nsp148D48NGatAat10261026All0.000196
ORF1ab/nsp148D48NGatAat703703B.1.1.70.000629
ORF1ab/nsp148D48DgaTgaC484484All0.000093
ORF1ab/nsp148D48VgAtgTt322322All0.000062
ORF1ab/nsp148D48VgAtgTt317317AY.1170.026792
ORF1ab/nsp148D48YGatTat226226All0.000043
ORF1ab/nsp148D48EgaTgaA75116All0.000014
ORF1ab/nsp148D48EgaTgaG41116All0.000008
ORF1ab/nsp148D48NGatAat110110AY.1030.000683
ORF1ab/nsp148D48GgAtgGt106106B.1.1770.001396
ORF1ab/nsp148D48delGAT---9898All0.000019
ORF1ab/nsp148D48DgaTgaC9595AY.250.000571
ORF1ab/nsp148D48YGatTat8989B.1.1.70.00008
ORF1ab/nsp148D48DgaTgaC8888AY.390.002175
ORF1ab/nsp148D48DgaTgaC6767B.1.1.70.00006
ORF1ab/nsp148D48GgAtgGt6161B.1.3510.001813
ORF1ab/nsp148D48GgAtgGt5858B.1.1.70.000052
ORF1ab/nsp148D48AgAtgCt5757All0.000011
ORF1ab/nsp148D48NGatAat5757B.1.5170.024338
ORF1ab/nsp148D48DgaTgaC4646AY.40.000072
ORF1ab/nsp148D48NGatAat3434AY.40.000053
ORF1ab/nsp148D48DgaTgaC2828AY.4.20.000607
ORF1ab/nsp148D48EgaTgaA2427AY.430.000106
ORF1ab/nsp148D48EgaTgaG327AY.430.000013
ORF1ab/nsp148D48delGAT---2727B.1.1.70.000024
ORF1ab/nsp148D48DgaTgaC2626B.1.617.20.000098
ORF1ab/nsp148D48GgAtgGt2222AY.440.000134
ORF1ab/nsp148D48NGatAat2020B.1.617.20.000075
ORF1ab/nsp148D48EgaTgaG1718AY.98.10.000823
ORF1ab/nsp148D48EgaTgaA118AY.98.10.000048
ORF1ab/nsp148D48GgAtgGt1717AY.40.000026
ORF1ab/nsp148D48NGatAat1616AY.430.000071
ORF1ab/nsp148D48EgaTgaA1616B.1.1.5230.025
ORF1ab/nsp148D48GgAtgGt1515AY.1020.000398
ORF1ab/nsp148D48DgaTgaC1515AY.440.000091
ORF1ab/nsp148D48YGatTat1414AY.200.000544
ORF1ab/nsp148D48NGatAat1313B.1.10.000236
ORF1ab/nsp148D48EgaTgaA1213B.1.1.70.000011
ORF1ab/nsp148D48EgaTgaG113B.1.1.70.000001
ORF1ab/nsp148D48GgAtgGt1313B.1.617.20.000049
ORF1ab/nsp148D48AgAtgCt1212AY.40.000019
ORF1ab/nsp148D48AgAtgCt1212B.1.1.70.000011
ORF1ab/nsp148D48GgAtgGt1111AY.1030.000068
ORF1ab/nsp148D48DgaTgaC1111AY.290.000174
ORF1ab/nsp148D48DgaTgaC1111B.1.1770.000145
ORF1ab/nsp148D48GgAtgGt1111B.1.258.140.010784
ORF1ab/nsp148D48YGatTat1111B.1.3110.00302
ORF1ab/nsp148D48YGatTat1010AY.40.000016
ORF1ab/nsp148D48DgaTgaC1010AY.910.007305
ORF1ab/nsp148D48YGatTat1010B.1.20.000088
ORF1ab/nsp148D48NGatAat99AY.1130.001282
ORF1ab/nsp148D48YGatTat99AY.250.000054
ORF1ab/nsp148D48DgaTgaC99AY.30.00009
ORF1ab/nsp148D48delGAT---99AY.40.000014
ORF1ab/nsp148D48YGatTat88AY.1030.00005
ORF1ab/nsp148D48YGatTat77AY.430.000031
ORF1ab/nsp148D48AgAtgCt77AY.440.000043
ORF1ab/nsp148D48NGatAat77AY.440.000043
ORF1ab/nsp148D48DgaTgaC77B.10.000064
ORF1ab/nsp148D48YGatTat77B.10.000064
ORF1ab/nsp148D48GgAtgGt77B.1.36.290.003621
ORF1ab/nsp148D48DgaTgaC66AY.1020.000159
ORF1ab/nsp148D48GgAtgGt66B.10.000055
ORF1ab/nsp148D48delGAT---66B.1.1770.000079
ORF1ab/nsp148D48AgAtgCt66B.1.617.20.000023
ORF1ab/nsp148D48EgaTgaG46B.1.617.20.000015
ORF1ab/nsp148D48EgaTgaA26B.1.617.20.000008
ORF1ab/nsp148D48NGatAat55AY.340.000485
ORF1ab/nsp148D48DgaTgaC55AY.430.000022
ORF1ab/nsp148D48DgaTgaC55AY.470.000156
ORF1ab/nsp148D48DgaTgaC55B.1.1.2280.015244
ORF1ab/nsp148D48DgaTgaC55B.1.20.000044
ORF1ab/nsp148D48AgAtgCt55B.1.20.000044
ORF1ab/nsp148D48HGatCat44All0.000001
ORF1ab/nsp148D48GgAtgGt44AY.1130.00057
ORF1ab/nsp148D48YGatTat44AY.1200.000149
ORF1ab/nsp148D48YGatTat44AY.230.000279
ORF1ab/nsp148D48GgAtgGt44AY.250.000024
ORF1ab/nsp148D48NGatAat44B.10.000037
ORF1ab/nsp148D48GgAtgGt44B.1.10.000073
ORF1ab/nsp148D48delGAT---44B.1.1.330.001684
ORF1ab/nsp148D48delGAT---44B.1.1.3480.003185
ORF1ab/nsp148D48EgaTgaA44B.1.20.000035
ORF1ab/nsp148D48DgaTgaC44B.1.2210.000284
ORF1ab/nsp148D48YGatTat44P.10.000063
ORF1ab/nsp148D48TGATACA33All0.000001
ORF1ab/nsp148D48DgaTgaC33AY.1030.000019
ORF1ab/nsp148D48DgaTgaC33AY.1210.000406
ORF1ab/nsp148D48delGAT---33AY.250.000018
ORF1ab/nsp148D48delGAT---33AY.4.50.000322
ORF1ab/nsp148D48GgAtgGt33AY.420.000121
ORF1ab/nsp148D48GgAtgGt33AY.430.000013
ORF1ab/nsp148D48delGAT---33AY.430.000013
ORF1ab/nsp148D48YGatTat33AY.440.000018
ORF1ab/nsp148D48YGatTat33AY.470.000093
ORF1ab/nsp148D48DgaTgaC33AY.90.000203
ORF1ab/nsp148D48GgAtgGt33B.1.2580.0002
ORF1ab/nsp148D48YGatTat33B.1.5260.000071
ORF1ab/nsp148D48GgAtgGt33C.370.000348
ORF1ab/nsp148D48EgaTgaG33Q.70.003333
ORF1ab/nsp148D48NGatAat33Q.80.000495
ORF1ab/nsp148D48del1gaTga-12All0
ORF1ab/nsp148D48del1Gat-at12All0
ORF1ab/nsp148D48GgAtgGt22AY.1000.000092
ORF1ab/nsp148D48EgaTgaG22AY.1020.000053
ORF1ab/nsp148D48NGatAat22AY.1170.000169
ORF1ab/nsp148D48delGAT---22AY.1200.000074
ORF1ab/nsp148D48GgAtgGt22AY.200.000078
ORF1ab/nsp148D48NGatAat22AY.200.000078
ORF1ab/nsp148D48NGatAat22AY.250.000012
ORF1ab/nsp148D48HGatCat22AY.250.000012
ORF1ab/nsp148D48GgAtgGt22AY.260.000057
ORF1ab/nsp148D48GgAtgGt22AY.30.00002
ORF1ab/nsp148D48NGatAat22AY.30.00002
ORF1ab/nsp148D48DgaTgaC22AY.3.10.000171
ORF1ab/nsp148D48GgAtgGt22AY.330.000134
ORF1ab/nsp148D48DgaTgaC22AY.39.10.000079
ORF1ab/nsp148D48GgAtgGt22AY.4.10.001475
ORF1ab/nsp148D48YGatTat22AY.50.000041
ORF1ab/nsp148D48GgAtgGt22AY.750.000112
ORF1ab/nsp148D48EgaTgaA12AY.90.000068
ORF1ab/nsp148D48EgaTgaG12AY.90.000068
ORF1ab/nsp148D48AgAtgCt22B.10.000018
ORF1ab/nsp148D48EgaTgaA12B.10.000009
ORF1ab/nsp148D48EgaTgaG12B.10.000009
ORF1ab/nsp148D48YGatTat22B.1.10.000036
ORF1ab/nsp148D48EgaTgaA22B.1.177.860.000367
ORF1ab/nsp148D48NGatAat22B.1.20.000018
ORF1ab/nsp148D48YGatTat22B.1.2580.000133
ORF1ab/nsp148D48DgaTgaC22B.1.3510.000059
ORF1ab/nsp148D48delGAT---22B.1.36.170.000988
ORF1ab/nsp148D48DgaTgaC22B.1.36.20.021505
ORF1ab/nsp148D48YGatTat22B.1.4270.000101
ORF1ab/nsp148D48delGAT---22B.1.4270.000101
ORF1ab/nsp148D48YGatTat22B.1.4290.000047
ORF1ab/nsp148D48DgaTgaC22B.1.4290.000047
ORF1ab/nsp148D48EgaTgaA22B.1.4290.000047
ORF1ab/nsp148D48AgAtgCt22B.1.4290.000047
ORF1ab/nsp148D48NGatAat22B.1.4730.038462
ORF1ab/nsp148D48YGatTat22B.1.5970.009259
ORF1ab/nsp148D48delGAT---22B.1.617.20.000008
ORF1ab/nsp148D48YGatTat22B.1.617.20.000008
ORF1ab/nsp148D48GgAtgGt22C.10.004785
ORF1ab/nsp148D48delGAT---22N.40.008511
ORF1ab/nsp148D48delGAT---22N.60.014184
ORF1ab/nsp148D48NGatAat22P.1.70.000639
ORF1ab/nsp148D48delGAT---22P.20.000373
ORF1ab/nsp148D48NGatAat22R.10.00018
ORF1ab/nsp148D48delGAT---11A0.000387
ORF1ab/nsp148D48EgaTgaA11A.10.000282
ORF1ab/nsp148D48AgAtgCt11A.2.20.001689
ORF1ab/nsp148D48CGATTGC11All0
ORF1ab/nsp148D48LGATTTG11All0
ORF1ab/nsp148D48del2gATg--11All0
ORF1ab/nsp148D48YGatTat11AT.10.005128
ORF1ab/nsp148D48NGatAat11AY.1000.000046
ORF1ab/nsp148D48YGatTat11AY.1020.000027
ORF1ab/nsp148D48VgAtgTt11AY.1020.000027
ORF1ab/nsp148D48AgAtgCt11AY.1030.000006
ORF1ab/nsp148D48EgaTgaA11AY.1100.000113
ORF1ab/nsp148D48GgAtgGt11AY.1100.000113
ORF1ab/nsp148D48EgaTgaA11AY.1130.000142
ORF1ab/nsp148D48delGAT---11AY.1160.000287
ORF1ab/nsp148D48EgaTgaG11AY.116.10.000311
ORF1ab/nsp148D48NGatAat11AY.1190.000035
ORF1ab/nsp148D48delGAT---11AY.120.000316
ORF1ab/nsp148D48YGatTat11AY.120.000316
ORF1ab/nsp148D48EgaTgaG11AY.120.000316
ORF1ab/nsp148D48EgaTgaA11AY.1200.000037
ORF1ab/nsp148D48DgaTgaC11AY.160.00038
ORF1ab/nsp148D48YGatTat11AY.160.00038
ORF1ab/nsp148D48delGAT---11AY.230.00007
ORF1ab/nsp148D48DgaTgaC11AY.230.00007
ORF1ab/nsp148D48DgaTgaC11AY.260.000028
ORF1ab/nsp148D48DgaTgaC11AY.270.000065
ORF1ab/nsp148D48NGatAat11AY.270.000065
ORF1ab/nsp148D48AgAtgCt11AY.290.000016
ORF1ab/nsp148D48NGatAat11AY.290.000016
ORF1ab/nsp148D48DgaTgaC11AY.29.10.000221
ORF1ab/nsp148D48YGatTat11AY.30.00001
ORF1ab/nsp148D48EgaTgaG11AY.30.00001
ORF1ab/nsp148D48NGatAat11AY.3.10.000085
ORF1ab/nsp148D48GgAtgGt11AY.300.000396
ORF1ab/nsp148D48DgaTgaC11AY.34.10.00051
ORF1ab/nsp148D48YGatTat11AY.360.000148
ORF1ab/nsp148D48delGAT---11AY.380.000499
ORF1ab/nsp148D48NGatAat11AY.380.000499
ORF1ab/nsp148D48GgAtgGt11AY.390.000025
ORF1ab/nsp148D48NGatAat11AY.390.000025
ORF1ab/nsp148D48YGatTat11AY.39.20.001541
ORF1ab/nsp148D48NGatAat11AY.4.20.000022
ORF1ab/nsp148D48AgAtgCt11AY.4.20.000022
ORF1ab/nsp148D48YGatTat11AY.4.40.000409
ORF1ab/nsp148D48del1Gat-at11AY.4.50.000107
ORF1ab/nsp148D48AgAtgCt11AY.410.000294
ORF1ab/nsp148D48DgaTgaC11AY.420.00004
ORF1ab/nsp148D48AgAtgCt11AY.430.000004
ORF1ab/nsp148D48EgaTgaG11AY.440.000006
ORF1ab/nsp148D48YGatTat11AY.450.000122
ORF1ab/nsp148D48delGAT---11AY.460.000104
ORF1ab/nsp148D48NGatAat11AY.46.50.000198
ORF1ab/nsp148D48AgAtgCt11AY.50.000021
ORF1ab/nsp148D48GgAtgGt11AY.500.000799
ORF1ab/nsp148D48GgAtgGt11AY.540.000095
ORF1ab/nsp148D48NGatAat11AY.540.000095
ORF1ab/nsp148D48DgaTgaC11AY.590.00053
ORF1ab/nsp148D48EgaTgaG11AY.60.000041
ORF1ab/nsp148D48delGAT---11AY.60.000041
ORF1ab/nsp148D48GgAtgGt11AY.600.000938
ORF1ab/nsp148D48DgaTgaC11AY.610.000105
ORF1ab/nsp148D48EgaTgaA11AY.610.000105
ORF1ab/nsp148D48YGatTat11AY.680.000274
ORF1ab/nsp148D48GgAtgGt11AY.690.000187
ORF1ab/nsp148D48NGatAat11AY.7.10.000113
ORF1ab/nsp148D48YGatTat11AY.7.10.000113
ORF1ab/nsp148D48YGatTat11AY.750.000056
ORF1ab/nsp148D48DgaTgaC11AY.75.10.00018
ORF1ab/nsp148D48YGatTat11AY.75.10.00018
ORF1ab/nsp148D48delGAT---11AY.9.10.000084
ORF1ab/nsp148D48YGatTat11AY.9.20.000037
ORF1ab/nsp148D48delGAT---11AY.9.20.000037
ORF1ab/nsp148D48NGatAat11AY.9.20.000037
ORF1ab/nsp148D48EgaTgaG11AY.920.000287
ORF1ab/nsp148D48delGAT---11AY.950.000761
ORF1ab/nsp148D48DgaTgaC11AY.980.000079
ORF1ab/nsp148D48NGatAat11AY.99.20.000092
ORF1ab/nsp148D48NGatAat11B0.000128
ORF1ab/nsp148D48delGAT---11B.10.000009
ORF1ab/nsp148D48TGATACA11B.10.000009
ORF1ab/nsp148D48LGATTTG11B.10.000009
ORF1ab/nsp148D48DgaTgaC11B.1.10.000018
ORF1ab/nsp148D48EgaTgaA11B.1.10.000018
ORF1ab/nsp148D48GgAtgGt11B.1.1.10.000327
ORF1ab/nsp148D48delGAT---11B.1.1.1700.001247
ORF1ab/nsp148D48delGAT---11B.1.1.2320.001815
ORF1ab/nsp148D48NGatAat11B.1.1.2360.006897
ORF1ab/nsp148D48EgaTgaG11B.1.1.2580.003257
ORF1ab/nsp148D48AgAtgCt11B.1.1.2670.010101
ORF1ab/nsp148D48GgAtgGt11B.1.1.280.000311
ORF1ab/nsp148D48VgAtgTt11B.1.1.2990.012195
ORF1ab/nsp148D48DgaTgaC11B.1.1.3040.002809
ORF1ab/nsp148D48TGATACA11B.1.1.3060.000438
ORF1ab/nsp148D48delGAT---11B.1.1.3110.000358
ORF1ab/nsp148D48delGAT---11B.1.1.3220.008772
ORF1ab/nsp148D48NGatAat11B.1.1.3260.001761
ORF1ab/nsp148D48delGAT---11B.1.1.390.00051
ORF1ab/nsp148D48NGatAat11B.1.1.4860.001577
ORF1ab/nsp148D48DgaTgaC11B.1.1.5180.006329
ORF1ab/nsp148D48AgAtgCt11B.1.1.5190.00004
ORF1ab/nsp148D48YGatTat11B.1.1.5190.00004
ORF1ab/nsp148D48DgaTgaC11B.1.1.5190.00004
ORF1ab/nsp148D48DgaTgaC11B.1.1.630.000386
ORF1ab/nsp148D48del2gATg--11B.1.1.70.000001
ORF1ab/nsp148D48HGatCat11B.1.1.70.000001
ORF1ab/nsp148D48del1gaTga-11B.1.1.70.000001
ORF1ab/nsp148D48NGatAat11B.1.1600.000031
ORF1ab/nsp148D48GgAtgGt11B.1.1600.000031
ORF1ab/nsp148D48DgaTgaC11B.1.160.300.002976
ORF1ab/nsp148D48GgAtgGt11B.1.160.330.004425
ORF1ab/nsp148D48NGatAat11B.1.1770.000013
ORF1ab/nsp148D48delGAT---11B.1.177.110.001698
ORF1ab/nsp148D48EgaTgaA11B.1.177.110.001698
ORF1ab/nsp148D48YGatTat11B.1.177.120.000152
ORF1ab/nsp148D48delGAT---11B.1.177.160.000664
ORF1ab/nsp148D48NGatAat11B.1.177.500.004695
ORF1ab/nsp148D48delGAT---11B.1.177.510.004566
ORF1ab/nsp148D48AgAtgCt11B.1.177.540.000606
ORF1ab/nsp148D48GgAtgGt11B.1.177.690.001484
ORF1ab/nsp148D48NGatAat11B.1.177.730.000566
ORF1ab/nsp148D48delGAT---11B.1.177.870.000728
ORF1ab/nsp148D48delGAT---11B.1.177.90.001332
ORF1ab/nsp148D48delGAT---11B.1.1790.003759
ORF1ab/nsp148D48delGAT---11B.1.20.000009
ORF1ab/nsp148D48VgAtgTt11B.1.20.000009
ORF1ab/nsp148D48HGatCat11B.1.20.000009
ORF1ab/nsp148D48EgaTgaA11B.1.221.30.000833
ORF1ab/nsp148D48GgAtgGt11B.1.2290.009709
ORF1ab/nsp148D48EgaTgaG11B.1.2430.00007
ORF1ab/nsp148D48CGATTGC11B.1.2580.000067
ORF1ab/nsp148D48DgaTgaC11B.1.2580.000067
ORF1ab/nsp148D48YGatTat11B.1.3370.005587
ORF1ab/nsp148D48YGatTat11B.1.3510.00003
ORF1ab/nsp148D48DgaTgaC11B.1.360.000146
ORF1ab/nsp148D48YGatTat11B.1.4240.008065
ORF1ab/nsp148D48delGAT---11B.1.4290.000024
ORF1ab/nsp148D48NGatAat11B.1.438.10.000107
ORF1ab/nsp148D48NGatAat11B.1.4590.004082
ORF1ab/nsp148D48NGatAat11B.1.466.20.000391
ORF1ab/nsp148D48YGatTat11B.1.4870.019608
ORF1ab/nsp148D48GgAtgGt11B.1.5260.000024
ORF1ab/nsp148D48AgAtgCt11B.1.5260.000024
ORF1ab/nsp148D48NGatAat11B.1.5260.000024
ORF1ab/nsp148D48TGATACA11B.1.5380.008065
ORF1ab/nsp148D48EgaTgaA11B.1.5960.000087
ORF1ab/nsp148D48DgaTgaC11B.1.5960.000087
ORF1ab/nsp148D48YGatTat11B.1.5960.000087
ORF1ab/nsp148D48YGatTat11B.1.5990.00365
ORF1ab/nsp148D48VgAtgTt11B.1.6090.000479
ORF1ab/nsp148D48VgAtgTt11B.1.617.20.000004
ORF1ab/nsp148D48EgaTgaA11B.1.6370.000073
ORF1ab/nsp148D48delGAT---11C.350.000774
ORF1ab/nsp148D48YGatTat11D.20.000075
ORF1ab/nsp148D48EgaTgaG11D.20.000075
ORF1ab/nsp148D48NGatAat11P.10.000016
ORF1ab/nsp148D48GgAtgGt11P.10.000016
ORF1ab/nsp148D48DgaTgaC11P.10.000016
ORF1ab/nsp148D48DgaTgaC11P.1.120.000484
ORF1ab/nsp148D48NGatAat11P.1.140.000048
ORF1ab/nsp148D48YGatTat11P.1.150.000232
ORF1ab/nsp148D48YGatTat11P.20.000187
ORF1ab/nsp148D48NGatAat11Q.10.000126
ORF1ab/nsp148D48NGatAat11Q.30.000145
ORF1ab/nsp148D48YGatTat11R.10.00009

CoV-GLUE is still in development. CoV-GLUE was originally developed as part of COG-UK by Josh Singer using the GLUE framework at the MRC-University of Glasgow Centre for Virus Research (CVR). In 2021 it was redeveloped by Richard Orton to scale to the millions of genome sequences available. We acknowledge support from the MRC (MC_UU_12014/12), WT (220977/Z/20/Z) and UKRI G2P (MR/W005611/1). Please contact Richard Orton with any queries: Richard.Orton@glasgow.ac.uk

CoV-GLUE uses sequence data from GISAID. GISAID data provided via this web application is subject to GISAID's Terms and Conditions.